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KMID : 1123920070210030709
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Korean Journal of Oriental Physiology and Pathology
2007 Volume.21 No. 3 p.709 ~ p.713
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Effect of Chrysanthemum Morifolium Extracts on the Synthesis of Laminin of Madin-Darby Canine Kidney Cells
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Na Ho-jeong
Jeon So-ra
Cha Dong-suk
Eun Jae-Soon
Lim Jong-Pil
Shin Tae-Yong
Oh Chan-Ho
Yang Jae-Heon
Kim Dae-Keun
Leem Jae-Yoon
Chae Byeong-Suk
Kim Sung-Zoo
Jeon Hoon
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Abstract
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Basement membranes (BMs) are extracellular matrices associated with epithelia, endothelia, muscle, fat and peripheral nerve. They are involved in cell survival, migration, differentiation. BMs functions also include tissue formation and provide mechanical stability as a selective barriers. Laminins are heterotrimeric glycoproteins found in BMs and have a crucial role in cell adhesion and signalling. Madin-Darby canine kidney (MDCK) cells are the best established mammalian model for studying epithelial cell biology The cells form an epithelial monolayer, with tight junctions separating an apical surface from a basolateral membrane facing the filter support and neighboring cells. In this study, using MDCK cells, the synthesis of the BM protein such as laminin with or without methanol extract of Chrysanthemum morifolium (CM) stimulation was analyzed by immunoblotting and CM showed significant increased cell density and enhanced synthesis of laminin.
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KEYWORD
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Basement membranes (BMs), laminin, Chrysanthemum morifolium (CM), Madin-Darby canine kidney (MDCK) cells
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